ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (2): 204-210.doi: 10.11843/j.issn.0366-6964.2015.02.005

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Expression of IGF-I and MSTN in Taihu and Wanxi White Goose Breast Muscle and Their Correlation with Carcass Traits

TANG Qing-ping,SONG Chi,HU Yan,ZHANG Shuang-jie,ZHAO Dong-wei,ZOU Jian-min*   

  1. (Key Laboratory of Poultry Heredity & Breeding,Jiangsu Institute of Poultry Science,Yangzhou 225125,China)
  • Received:2013-11-29 Online:2015-02-23 Published:2015-02-23

Abstract:

The aim of this study was to investigate the effects of IGF-I and MSTN on goose skeletal muscle growth.In this study,the expression of IGF-I and MSTN in breast muscle of Taihu and Wanxi White goose at the age of 70 days were detected with Multiplex Competitive Fluorescent-PCR and Enzyme-linked immunoassay (ELISA) methods,and the correlations between gene expression levels and carcass traits were investigated.The result showed that the body weight and breast muscle weight of Taihu goose were significantly lower than those of Wanxi White goose at the age of 70 days,there was no significant gender difference in breast muscle percentage between the two breeds and there was significant gender difference in body weight and breast muscle weight between the two breeds.There was no breed and gender difference at the mRNA and protein levels of IGF-I and MSTN,and but in Taihu goose,IGF-I mRNA expression in male breast muscle was significantly higher than that of females.There was no significant correlation between mRNA expression and protein content of the two genes.There was a positive correlation between IGF-I and MSTN at the protein level of breast muscle.There was a negative correlation between IGF-I mRNA expression and body weight,breast muscle weight and breast muscle percentage in goose,and a negative correlation between the expression of MSTN and body weight had been detected in Wanxi White goose.The results suggested that the expression levels of genes involving in positive and negative regulation to muscle growth may be in a dynamic balance,special participated in the growth regulation on Wanxi White goose at the age of 70 days.

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